Figure 6. NGF does not modulate NK cell surface marker expression.

Splenocytes were cultured during three days in the presence of IL-2 (1000 U/ml) and NGF at different concentrations. Then NK cells (CD3-NK1.1+) were analysed by flow cytometry for the expression of the surface markers indicated on the X-axis. Dead cells were excluded by staining with Live Dead cell marker. There were no significant differences between the two culture conditions for any of the markers tested (p>0.05). The data represent the mean ±SEM of three different experiments.