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. 2010 Dec 1;5(12):e15053. doi: 10.1371/journal.pone.0015053

Figure 7. NGF reduces NK cell degranulation but does not influence IFNγ production.

Figure 7

A and B: Fresh splenocytes were assessed for degranulation (A), reflected by the percentage of CD107a+ NK cells (CD3-NK1.1+), and for cytokine production (B), reflected by the percentage of IFNγ+ NK cells (CD3-NK1.1+). C and D: the same experiment was performed on NK cells (CD3-NK1.1+) cultured for three days in the presence of IL-2 alone (open histograms) or of IL-2+ NGF (100 ng/ml, filled histograms). The white bars correspond to cells cultured during three days with IL-2 alone, and the grey bars correspond to cells cultured with IL-2+ NGF during the same period. On day three, cells from both culture conditions were incubated during five hours in the different conditions shown on the X-axis. Dead cells were excluded by staining with Live Dead cell marker. NGF inhibits degranulation to a certain extent in the condition IL-2+ IL-12+ IL-18+ NGF. The data shown are the means ± SEM of three experiments.