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Supernatants from purified NK cells activated for three days with IL-2 alone or with IL-2+ LPS are harvested and tested for NGF by ELISA. The negative control is culture medium (DMEM +10% FCS) and the positive control is BALF from a Balb/c mouse with allergic airway inflammation (induced by ovalbumin). The data shown are the means ± SEM of three experiments.
