Figure 6. Flow cytometry profile of NK cells with allo-specific monoclonal antibody PK136.
CD3- and CD19- splenocytes were assessed for expression of NKp46 (expressed by all NK cells in all mouse strains, y-axis) and the NK1.1 epitope detected by monoclonal antibody PK136 (x-axis). Phycoerythrin (PE)-conjugated anti-NKp46, allophycocyanin (APC)-anti-NK1.1, fluorescein isothiocyanate (FITC)-conjugated anti-CD3, and FITC-anti-CD19 were used to stain the indicated mouse strains. The percentage of NK1.1+ cells among NKp46+ CD3− CD19− cells is shown in each dot plot.