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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Arthritis Rheum. 2010 Dec;62(12):3706–3715. doi: 10.1002/art.27733

Figure 2.

Figure 2

Combination of IGF-1 and BMP7 exerts a compensatory anabolic gene expression effect. NP cells isolated from bovine IVD were cultured in a monolayer in 12-well plates at 8×105 cells/cm2. NP cells were stimulated in serum-free medium (control) or the control medium plus 100 ng/ml IGF-1, 100 ng/ml BMP7, cocktail of IGF-1 and BMP7, and 10 ng/ml IL-1α for 24 hrs. After stimulation, cells were harvested and the total RNA was extracted to perform real-time PCR of aggrecan (A) and collagen type-II genes (B). Error bars represent three different donors in three separate experiments. Analysis of variance was performed using StatView 5.0 software (SAS Institute, Cary, NC). P-values lower than 0.05 were considered significant. Six independent experiments in triplicates were performed.