Table 1.
Real-Time PCR Primer sequence.
| Gene | Primer Seq. | Annealing Tm | NCBI Accession No. |
|---|---|---|---|
| Aggrecan | Forward: 5’-TGAAACCACCTCCACCTTCCATGA-3’ Reverse: 5’-TCAAAGGCAGTGGTTGACTCTCCA-3’ |
55°C | NM_173981.2 |
| Collagen Type II | Forward: 5’-TCATAAGGATGTGTGGAAGCCCGA-3’ Reverse: 5’-GGCTGAGGCAGTCTTTCATGTCTT-3’ |
55°C | AY743675.1 |
| SOX-9 | Forward: 5’-ACCATGTCCGAGGACTCTGC-3’ Reverse: 5’-AACTTGTCCTCCTCGCTCTCCTTCTT-3’ |
55°C | AF278703 |
| Noggin | Forward: 5’-AGCGAGATCAAAGCGCTGGAGTT-3’ Reverse: 5’-TCTGTAACTTCCTCCGCAGCTTCT-3’ |
55°C | XM_582573.4 |
| β-actin | Forward: 5’-AAGAGATCAATGACCTGGCACCCA-3’ Reverse: 5’-ACTCCTGCTTGCTGATCCATCT-3’ |
55°C | BC142413.1 |