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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Genesis. 2010 Nov;48(11):667–672. doi: 10.1002/dvg.20669

FIG. 2.

FIG. 2

Foxn4-expressing cells give rise to all interneurons of the three known V2 lineages. (a-p) Spinal cord sections from E14.5 (a-h) and E12.5 (i-p) Foxn4-Cre;R26R-YFP embryos were stained by double-immunofluorescence using the indicated antibodies. Sections in (a,e,i,m) were also weakly counterstained with DAPI. Note the colocalization between YFP and Chx10 in V2a neurons (a-d), between YFP and Gata2 in V2b neurons (e-h), and between YFP and Sox1 in V2c neurons (indicated by arrows) (i-l). YFP and Isl1/2 are not colocalized in motor neurons (m-p). The dashed ovals in (i) outline the regions where Sox1-immunoreactive V2c cells are located. CC, central canal; VLF, ventrolateral funiculus; VZ, ventricular zone. Scale bar equals 50 μm (a,e,i,m), 12.5 μm (b-d,f-h,j-l), and 12 μm (n-p).