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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Micron. 2010 Jul 16;42(2):107–116. doi: 10.1016/j.micron.2010.07.004

Figure 5.

Figure 5

Three-dimensional models from single particle cryo-EM of a detergent-solubilized protein complexes can show density from the detergent micelle. A, A map from single particle analysis of dodecyl maltoside (DDM) solubilized Thermus thermophilus V-type ATPase (Lau and Rubinstein, 2010) resolved the two different hydrophobic subunits, I and L, from the detergent micelle used to keep the complex soluble. A 3D model depicting segmented subunits and subcomplexes shows a segment corresponding to the DDM micelle in semi-transparent dark grey near the bottom of the model. The other segments depict the A-subunits (yellow), B-subunits (red), the D and F subunit (dark blue), the C-subunit (light blue), the membrane bound I-subunit with the peripheral stalks consisting of subunits E and G (green), and the ring of L-subunits (pink). B, A cross section through the region of the complex usually embedded in the membrane resolves the ring of L-subunits, the I-subunit, and the micelle of DDM. The scale bars in both parts A and B represent 50 Å.