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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Hepatology. 2010 Apr;51(4):1391–1400. doi: 10.1002/hep.23431

Fig. 2. Notch receptor mRNA expression increases as Notch signaling dosage decreases at P15.

Fig. 2

The relative expression ratio of Notch receptors was calculated using real-time PCR efficiency and the deviation in cycle threshold (Ct) of experimental versus control [without Alb-Cre] samples, expressed in comparison to HPRT mRNA levels. (A) In N2 KO, there are no significant changes in receptor mRNA. (B) In N1/N2 DKO there is significantly higher N3 and N4 mRNA being transcribed. (C) In RPB KO, all Notch receptors are significantly up-regulated, suggesting a feed-forward loop attempting to compensate for the loss of Notch signaling. Despite increased receptor expression, without RBP-JK, Notch receptors cannot activate downstream targets. Statistical analysis was performed using a one-way ANOVA with Tukey’s Multiple Comparison Test. Error bars are standard error of the mean. *P<0.05.