Figure 3.
STR profile of CGL1. This STR profile example featuring CGL1 reveals the complexities, such as polyploidy, associated with hybrid cell lines. The STR procedure follows: Cell pellets (~3.5 × 105 cells each) were resuspended in 200 μL Qiagen Buffer G2. DNA extraction and purification were conducted on the EZ1 Advanced robotic system (Qiagen, Germantown, MD); readings on the NanoDrop ND-1000 Spectrophotometer (Thermo, Wilmington, DE) produced DNA concentrations. After normalizing the DNA, 1.0 μL of sample was amplified using PowerPlex 1.2 (Promega, Madison, WI) in an 8.0-μL reaction. An aliquot of the product was mixed with Hi-Di formamide [Applied Biosystems Inc. (ABI), Foster City, CA] and Internal Lane Standard (CXR) 60–400 bases (Promega), denatured, and then fractionated on an ABI 3130xl Genetic Analyzer. Resulting data were processed and evaluated using ABI Genemapper v3.2.1.