Figure 4.

Effect of hemichannel blockers on ischaemia-evoked permeability of the pyramidal cell membrane to propidium. (A) Propidium fluorescence (F, normalized to the value at start of ischaemia) in area CA1 of hippocampal slices to which ischaemia solution was applied, with and without pannexin blockers [eight slices without blockers (con), five with carbenoxolone (Cbx) 100 µM and six with mefloquine (Mfq) 25 µM]. When used, the blockers were present throughout the whole experiment. P_Cbx-values compare the carbenoxolone data with the control condition. (B) Specimen images from one slice at the start of ischaemia (t = 0) and after 90 min ischaemia.