. 2010 Nov 30;2010:253983. doi: 10.1155/2010/253983

Table 2.

Screening the expression of soluble IgA1 proteases by ELISA.

	Expression level (OD450)
	First round screening		Second round screening		Third round screening
Control					Negative control: C41(DE3) supernatant	0.08
			Positive control: purified IgA1 protease	0.21	Positive control: purified IgA1 protease	0.20
	*Negative control: BL21(DE3) supernatant*	*0.10*	Negative control: BL21(DE3) supernatant	0.08

Construct

	*C41(DE3)*	*1.13*	0.4 mM IPTG, 12°C	0.72	15°C, 0.2 mM IPTG	0.57
					15°C, 0.4 mM IPTG	0.72
			0.4 mM IPTG, 20°C	1.10	15°C, 0.6 mM IPTG	0.54
					20°C, 0.2 mM IPTG	0.87
			1 mM IPTG, 12°C	0.66	20°C, 0.4 mM IPTG	1.17
					20°C, 0.6 mM IPTG	1.02
			1 mM IPTG, 20°C	1.01	26°C, 0.2 mM IPTG	0.57
					26°C, 0.4 mM IPTG	0.51
					26°C, 0.6 mM IPTG	0.33

	*Origami B(DE3)*	*0.98*	0.4 mM IPTG, 12°C	0.36
			0.4 mM IPTG, 20°C	0.29
			1 mM IPTG, 12°C	0.35
			1 mM IPTG, 20°C	0.29

	*BL21(DE3)*	*0.93*	0.4 mM IPTG, 12°C	0.54
			0.4 mM IPTG, 20°C	0.59
pET-IGAN			1 mM IPTG, 12°C	0.49
			1 mM IPTG, 20°C	0.61

	*Tuner (DE3)*	*0.92*	0.4 mM IPTG, 12°C	0.48
			0.4 mM IPTG, 20°C	0.67
			1 mM IPTG, 12°C	0.46
			1 mM IPTG, 20°C	0.57

	*BL21(DE3)pGro7*	*1.01*	0.4 mM IPTG, 12°C	0.54
			0.4 mM IPTG, 20°C	0.73
			1 mM IPTG, 12°C	0.61
			1 mM IPTG, 20°C	0.47

	*ArcticExpress (DE3)*	*1.00*	0.4 mM IPTG, 12°C	0.68
			0.4 mM IPTG, 20°C	0.98
			1 mM IPTG, 12°C	0.70
			1 mM IPTG, 20°C	0.89
	*KRX*	*0.56*
	*Origami B(DE3)pLysS*	*0.75*
	*C43 (DE3)*	*0.14*

pET-S-IGAN	*BL21(DE3)*	*0.90*
	*C41(DE3)*	*0.83*
	*C43(DE3)*	*0.20*
	*Origami B(DE3)*	*0.82*
	*KRX*	*0.73*
	*ArcticExpress(DE3)*	*0.85*
	*BL21(DE3)pGro7*	*0.86*
	*Tuner (DE3)*	*0.82*

pCold-S-IGAN	*BL21(DE3)*	*0.76*
	*C41(DE3)*	*0.70*
	*C43(DE3)*	*0.79*
	*Origami B(DE3)*	*0.70*
	*BL21(DE3)pGro7*	*0.44*

pCold-IGAN	*BL21(DE3)*	*0.56*
	*C41(DE3)*	*0.68*
	*C43(DE3)*	*0.15*
	*Origami B(DE3)*	*0.24*
	*BL21(DE3)pGro7*	*0.28*