Figure 7.

Effect of nutrient depletion on glutathione metabolism. The major primary thiol was measured in the wild type (GSH; solid line, circles) and ΔgshB::GmR (γ-EC; dashed line, squares) over the course of 12 d in deplete (shaded) and replete (white) conditions. Cells were precultured in BG11 medium (0 d) prior to transfer to BG11 (A) or BG11 lacking nitrate (B), sulfate (C), or phosphate (D) for 6 d. After 6 d of growth in deplete conditions, cells were transferred to fresh BG11 medium and grown for an additional 6 d. Error bars represent se of two measurements each from two independent cultures.