Table I. Products of sesquiterpene synthases expressed in E. coli.
Clone Name | Total Products | Kinetic Parameters | ||||
α-Guaiene | α-Humulene | δ-Guaiene | kcat | Km | kcat/Km | |
% | s−1 | μm | s−1 mm−1 | |||
AcC2 | 18.1 | 0.7 | 81.2 | 4.99 × 10−3 | 2.71 | 1.836 |
AcC3 | 44.6 | 1.7 | 53.7 | 9.72 × 10−5 | 0.51 | 0.189 |
AcC4 | 20.9 | 0.9 | 78.2 | 7.34 × 10−3 | 3.05 | 2.404 |
AcL154’ | 20.8 | 0.7 | 78.5 | 1.58 × 10−5 | 0.45 | 0.035 |