Figure 9.

Transgenic rice plants overexpressing BERF1 and BEIL1. A and B, BERF1 and BEIL1 top overexpressor plants (T1 generation, pMO11 and pMO8 lines, respectively) compared with wild-type (wt) rice (Nipponbare). C, Five-day-old seedlings from Nipponbare and T2 segregation progeny of BEIL1 overexpression plants (pMO8). Bars = 1 cm. D, Relative expression levels of endogenous OsERF72, OsEREBP1, and OsACO1 genes in BERF1 expressors (T2 generation). Total RNA was extracted from a pool of five 10-d-old seedlings. The ratio between each gene level and the OsUBQ gene level obtained from wild-type plants was arbitrarily set at 1. Nipponbare was used as a wild-type control. E, Relative expression levels of the exogenous BEIL1 gene and endogenous OsEIL1, OsEBP89, and OsACO1 genes. Total RNA was extracted from a pool of five 7-d-old seedling leaves of BEIL1 overexpression plants (T2 generation). The ratio between each gene level and the OsUBQ gene level obtained from mild overexpressor plants (pMO8.15) was arbitrarily set at 1. F, Relative expression levels of endogenous OSH1, OSH15, and OsACO1 genes in 1-week-old wild-type seedlings. Total RNA was extracted from a 1-cm region of the stem containing the SAM and the first true leaf from a pool of five Nipponbare plants. qRT-PCR was performed in triplicate, and mean values with sd are shown. Two independent biological replicates gave similar results. [See online article for color version of this figure.]