Table 1.

Study design of preliminary skin permeation of LHCl through rat skin

Study code	Vehicle in donor compartment	Vehicle in receptor compartment	Penetration enhancer concentration (% w/v)
PS1	Distilled water	IPB of pH 7.4	None
PS2	IPB of pH 7.4	IPB of pH 7.4	None
PS3	Distilled water	IPB of pH 7.4	aDMF (5%)
PS4	Distilled water	IPB of pH 7.4	bDMSO (5%)
PS5	Distilled water	IPB of pH 7.4	2-Pyrrolidone (5%)
PS6	Ethanolic IPB of pH 7.4 (1:9)	IPB of pH 7.4	None
PS7	Ethanolic IPB of pH 7.4 (1:9)	IPB of pH 7.4	Turpentine Oil (5%)
PS8	Ethanolic IPB of pH 7.4 (1:9)	IPB of pH 7.4	Pine Oil (5%)
PS9	Ethanolic IPB of pH 7.4 (1:9)	IPB of pH 7.4	Menthol (5%)
PS10*	Distilled water	IPB of pH 7.4	None
PS11*	IPB of pH 7.4	IPB of pH 7.4	None
PS12	Distilled water	IPB of pH 7.4	DMSO (7.5%)
PS13	Distilled water	IPB of pH 7.4	DMSO (10%)

^*Drug concentration in donor compartment = 200 μg/ml

^aDMF = Dimethyl Formamide

^bDMSO = Dimethyl Sulfoxide. Drug concentration in donor compartment = 100 μg/ml