Figure 3.

Cells expressing FRS2α or FRS2α-8V show radial glial properties. (A): Most GFP-positive cells (green), which express FRS2α or FRS2α-8V, also expressed nestin (magenta), as indicated by the arrowheads. Scale bar = 50 μm. (B): The cell bodies of most GFP-positive cells in the S-phase (30 minutes after BrdU administration), identified by BrdU signals (magenta), were located at the basal half of VZ (arrowhead). In contrast, the cell bodies of GFP-positive cells in the G1 phase (12 hours after BrdU administration) were present at both the basal and apical (arrow) halves of VZ. Scale bar = 10 μm. (C): Quantification of the data presented in (B). About 300 cells were counted in each condition in 20–32 randomly selected areas. Abbreviations: BrdU, Bromodeoxyuridine; GFP, Green Fluorescent Protein; FRS2α, FGF receptor substrate 2α; VZ, ventricular zone.