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. Author manuscript; available in PMC: 2010 Dec 2.
Published in final edited form as: Am J Physiol Gastrointest Liver Physiol. 2005 Oct 6;290(2):G404–G416. doi: 10.1152/ajpgi.00344.2005

Fig. 3.

Fig. 3

p53 status does not affect IGFBP-3 induction by GF deprivation. Subconfluent EPC2-hTERT cells expressing the dominant negative form of p53 (p53R175H) or empty vector-transduced control (puro) cells were subjected to GF deprivation with KBM for the indicated time periods. Expression of IGFBP-3 and p53 in cell lysates was determined by Western blot analysis. β-Actin was used as a loading control. This result was reproducible in at least 2 independent experiments.