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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Ultrasound Med Biol. 2010 Nov;36(11):1907–1918. doi: 10.1016/j.ultrasmedbio.2010.05.014

Fig. 3.

Fig. 3

Flow cytometric analysis of HEK-293 viability and GFP expression. (a) Characteristic GFP intensity histogram on viable HEK-293 cells showing (1) minimal background fluorescence for untreated cells (unfilled black curve); (2) GFP expression in cells treated by Lipofectamine (open red curve); and (3) sonoporation with MBs (filled curve). Characteristic viability plots: (b) untreated healthy stained cells, (c) Cells transfected with pMax-GFP by Lipofectamine-2000 or (d) by sonoporation with MBs (2.0 W/cm2, duty cycle of 25%, 4 × 107 neutral MBs per mL and 3.2 μg/mL of pMax-GFP plasmid).