Fig. 7.

Effect of L-glutamate on [Ca²⁺]_i and Na⁺/Ca²⁺ exchange. Intracellular calcium ([Ca²⁺]_i) was measured with ratiometric live cell fluorescent imaging of Fura-2-AM in cultured astrocytes. The sodium-calcium exchanger (NCX) was inhibited in the reverse direction with 100 nM KB-R7943 before L-glutamate application. Pre-glutamate application of KB-R7943 did not reduce [Ca²⁺]_i in 100 μM condition. After 1 mM L-glutamate application, KB-R7943 reduced [Ca²⁺]_i at some time-points (*). Pre-administration of KB-R7943 did not significantly reduce propidium iodide (PrI) uptake in either condition, although there was a nonsignificant trend (P = 0.052) toward reduction after 1 mM glutamate application. (n = 3 separate experiments).