Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Dec 2;5(12):e14198. doi: 10.1371/journal.pone.0014198

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Le Blastier et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A) Alkaline phosphatase assays on concentrated supernatants. Specific alkaline phosphatase activity was measured by degradation of 5 mg/ml p-nitrophenyl phosphate (PNPP) in 100-fold concentrated supernatants from CB15N (WT), CB15N ΔelpS (ΔelpS), CB15N ΔelpS/pMR10 (ΔelpS/pMR10) and CB15N ΔelpS/pMR10-elpS (ΔelpS/pMR10-elpS) grown in poor medium. (B) Absence of detection of MreB in concentrated supernatants. Immunoblot with anti-MreB antibodies was done on cellular extracts and 100-fold concentrated supernatants from strains tested in (A) grown in poor medium. Proteins contained in samples tested in (A) were detected by electrophoresis and silver nitrate staining as a loading control for immunoblot.