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. 2010 Dec 2;6(12):e1001226. doi: 10.1371/journal.pgen.1001226

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Colony morphologies, quantifications of asexual spore (conidia, in light) and fruiting body (cleistothecia, in dark) formations of (A4) veA+, (A26) veA1, laeAΔ/veA+, laeAΔ/veA1, veAΔ, laeAΔ/veAΔ strains grown on the plates at 37°C for 5 days in the light asexually or in the dark sexually. For the quantification of conidia or cleistothecia, the 5×10 mm² sectors from 5 independent plates were used and the standard deviations are indicated as vertical bars. veA+ strains conidiation and cleistothecia levels were used as standard (100%). (B) The secondary metabolite sterigmatocystin (ST) production levels of the strains from (A) examined by TLC. 5×10³ conidia were point-inoculated at the center of the plates that were kept either in white light (90 µWm²) or in dark.