Figure 6.

ABCB1/PgP transporter does not mediate ZNF217-induced resistance to paclitaxel. (A) ABCB1 protein levels were analyzed by flow cytometry in control K562-R7, MDA-MB-231/pcDNA6, ZNF217-1 and ZNF217-2 cells. Representative FACS histograms of the three cell lines after incubation with or without ABCB1 antibody were superimposed according to increased PE fluorescence. (B) Intracellular DNR efflux was assessed by flow cytometry. Maximal DNR accumulation (100%) is represented by the DNR fluorescence median after 30 min accumulation. After DNR removal, cells were incubated 1 h in the absence (white columns) or in the presence of CSA (black columns). Results are means ± s.d. from three independent experiments.