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. 2010 Nov 11;10:284. doi: 10.1186/1471-2180-10-284

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Copyright ©2010 Janczarek et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Developing stages of biofilm formation in R. leguminosarum bv. trifolii wild type 24.2, rosR mutant Rt2472 and Rt2472(pRC24) strains observed after 2 and 4 days. The rosR mutant Rt2472 did not form typical biofilm after 4 days and was restored to the wild type phenotype after introduction of the rosR gene cloned on pRC24 plasmid. Top panel shows 4 dpi biofilms stained with Calcofluor, and the remaining panels show horizontal projected images from 2 and 4 dpi biofilms, with live (Syto-9, green fluorescence) and dead (propidium iodide, red fluorescence) cells. The insets show details of individual stages of biofilm formation.