Table 2.

Sensitivity of R. leguminosarum bv. trifolii rosR mutants to detergents, ethanol, and osmotic stress.

Strain	Minimal inhibitory concentration			Hyperosmotic	Hypo-osmotic
	SDS (% w/v)	DOC (% w/v)	Ethanol (%v/v)	stress (%)*	stress (%)*
Rt24.2	0.05 ± 0.005	0.10 ± 0.005	4.5 ± 0.28	77.1	51.6
Rt2440	0.02 ± 0.003†	0.030 ± 0.003†	2.3 ± 0.25†	11.5†	13.0†
Rt2441	0.02 ± 0.002†	0.030 ± 0.003†	3.0 ± 0.28	11.9†	15.2†
Rt2472	0.015 ± 0.002†	0.025 ± 0.002†	2.6 ± 0.28†	10.4†	13.3†

* - Strains were grown in TY supplemented with 85 mM NaCl (hyperosmotic) or GYM medium (hypo-osmotic) supplemented with Dilworth's vitamins for 2 days, and the growth was compared with that of strains grown in TY medium. OD₆₀₀ values were measured. Percentage growth values are the mean and SD from three independent trials.

† Difference between the wild type and the rosR mutants is statistically significant at P < 0.05 (Student's t test).