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Quantitative murein hydrolase assays of S. epidermidis 1457ΔlytSR. Aliquots (100 μg) of the extracellular proteins concentrated by ultrafiltration from the supernant were added to a 1-mg/ml suspension of M. luteus (A) and S. epidermidis (B) cells separately, and the turbidity at 600 nm was monitored for 4 h. Cell wall hydrolysis was determined by measurement of turbidity every 30 min. Data are means ± SD of 3 independent experiments.
