Table 4.
Primers used in this study
| Primers | Sequence(5'→3')* | Restriction |
|---|---|---|
| Primers used for PCR products in allelic gene replacement | ||
| lyt-UF (upstream fragment) | CCGGAATTCGAACCGATGGACCAGTAG | BamHI |
| lyt-UR (upstream fragment) | CGGAATTCTAAAGAGGGACGACAATGG | EcoRI |
| lyt-DF (downstream fragment) | CCCAAGCTTCAACAACTCGGTCTTCAA | HindIII |
| lyt-DR (downstream fragment)) | CTAGCTAGCAAAGGTATGGGAATGACG | NheI |
| Primers used in complementation of 1457ΔytSR1 strain | ||
| lyt-CF | GGGGTACCTTATTGAAGACCGAGTTGTTGTTTA | BamHI |
| lyt-CR | CGGGATCCTATGAAACAAGCCAATGTAAGTGC | KpnI |
| Primers used for real time RT-PCR in confirmation of microarray data | ||
| gyrB-RF | TTTCACTTTCTTCAGGGTTCTTAC | |
| gyrB-RR | CCATCTGTAGGACGCATTATTG | |
| lrgA-RF | GCATTGTGAAATTAGGTCAAGTTG | |
| lrgA-RR | ACTAATAATTGTGACGCAAAGCC | |
| serp2169-RF | GCATCCGCTTCTCCAATATCTG | |
| serp2169-RR | TAAACAACATACACACGCTAAACC | |
| ebsB-RF | TTTGATGCTGCGACTAAAGG | |
| ebsB-RR | CATTGCTGCCCATTCTGC | |
| arcA-RF | GGCTGACTCATACATCTTGG | |
| arcA-RR | GGGTTGTGGTGACATACG | |
| leuC-RF | CCAGGATGTTCTATGTGCTTAGG | |
| leuC-RR | CGCCTTTGCCTTGTCTTCC | |
* Primers were designed according to the genomic sequence of S. epidermidis RP62A (GenBank accession number CP000029).