Table 3.
List of primers and reference genes (names, function and amplicon size) under investigation. The primers for the gene of interest (COI) are also included.
| Gene | Forward primer | Reverse Primer | Function | Product size (bp) |
|---|---|---|---|---|
| 18S | 5'-GGTTTTCGGAACACGAGGTA-3' | 5'-TGGCATCGTTTATGGTCAGA-3' | Small ribosomal subunit | 200 |
| α Tubulin | 5'-CCATACCCTTCACCGACGTA-3' | 5'-AGGTGGGCATCAACTACCAG-3' | Structural constituent of cytoskeleton | 190 |
| Histone H3.3 | 5'-AGAGTGCTCCCTCAACTGGA-3' | 5'-GTCCTCAAAGAGACCCACCA-3' | To mark active chromatin (nucleosome structure) | 194 |
| Elongation Factor 1α | 5'-GCCCTTGAACCACTTCATGT-3' | 5'-ATCATCGGCGTCAACAAGAT-3' | Translational elongation | 195 |
| Elongation Factor 2 | 5'-ACGCATGTTCTCCTCACACA-3' | 5'-CGCTACCTGGTGGACAACTT-3' | Translational elongation | 198 |
| Calmodulin | 5'-CACCGTTCGTTTCATCCATA-3' | 5'-GTTCTGTCCCAGCGACCTC-3' | Calcium-binding protein | 182 |
| COI | 5'-GGGGGAGGAGACCCTATTCT-3' | 5'-ATGGTGGGCCCATACAATAA-3' | Energy production (electron transport chain) | 204 |