Fig. 3.

Nanodomain formation in heat-killed cells and V326N mutant cells. (A and F) AFM topographic images (scale bars: 2 μm), in buffer, showing two heat-killed (60 °C, 30 min) cells expressing wild-type Als5p (A) and two cells expressing the V5-tagged Als5p mutant protein Als5p^V326N bearing a single-site mutation in the amyloid region (F). (B–D and G–I) Sets of consecutive adhesion force maps (1 × 1 μm) recorded with an anti-V5 tip on the same target areas (map 1 and map 1′), followed by third maps recorded on remote areas (map 2). (E and J) Adhesion force histograms corresponding to the data (n = 1,024) shown in map 1.