Skip to main content
. 2010 Nov 12;107(48):20686–20690. doi: 10.1073/pnas.1010950107

Fig. 3.

Fig. 3.

BHA, but not O-MeBHA, enhanced the hydrolysis of TCBQ and TrCBQ-OH to DDBQ in a concentration- and pH-dependent manner. (A) BHA enhanced TCBQ hydrolysis in a concentration-dependent manner. (B) BHA enhanced TrCBQ-OH hydrolysis in a concentration-dependent manner. (C) BHA enhanced TCBQ hydrolysis in a pH-dependent manner; TCBQ, 0.05 mM; BHA, 0.20 mM. (D) O-MeBHA could not enhance TCBQ hydrolysis. All incubation mixtures contained the indicated concentration of BHA in phosphate buffer (10 mM, pH 7.0) at room temperature. The reactions were initiated by the addition of TCBQ or TrCBQ-OH (0.05 mM), followed by rapid mixing. DDBQ formation was monitored at 332 nm for 30 min.