Fig. 5.

Apoptosis in knockdown cell lines. Knockdown cell lines expressing CrkI were prepared as in Fig. 2. Immunoblotting analysis was performed using cleaved caspase-3 (Asp175) antibody on extracts from different knockdown cell lines either treated with doxorubicin (16 h, 2 µM) (a) or left untreated (b). Cleaved caspase-3, a biochemical apoptotic marker, was detected as a 17–19 kDa doublet. Representative results of two independent experiments are shown.