Skip to main content
. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Oncogene. 2010 Aug 23;29(48):6378–6389. doi: 10.1038/onc.2010.369

Fig. 6.

Fig. 6

Effect of knockdown on downstream signaling pathways. Knockdown cell lines expressing CrkI were prepared as in Fig. 2, and total cell lysates subjected to immunoblot analysis with phosphospecific antibodies for JNK (phospho-JNK), Erk1/2 (phospho-Erk) and Akt (phospho-Akt). The same blots were then re-probed with anti-JNK, anti-Erk or anti-Akt antibodies. Arrows show p54/p46 JNK and p44/p42 Erk proteins. Numbers below phospho-JNK, phospho-Erk and phospho-Akt blots are relative values normalized to total JNK, Erk and Akt levels, and represent the average of 3 independent experiments.