Rat primary TGNs (day 4 in culture) were stimulated for 1 hr with capsaicin (CAP, 100 nmol/L), then the media were assayed for substance P release by ELISA. 30 min pre-treatment with the EETs antagonist 14,15-EEZE (10 μmol/L) blocked capsaicin-stimulated substance P release (*P < 0.05, t-test, treatment vs. capsaicin alone; n=4). Capsaicin stimulation was carried out in the continued presence of 14,15-EEZE.