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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Nat Cell Biol. 2010 Nov 21;12(12):1154–1165. doi: 10.1038/ncb2119

Fig. 2. IRGM co-fractionates with mitochondria and localizes to their inner membrane or matrix.

Fig. 2

a. Purified mitochondria were untreated or subjected to osmotic shock (OS), or total protein solubilized with TX-100, and preparations digested with Proteinase K (Pr.K) and analyzed by immunoblotting. b. Mitochondrial preparations as in e were subjected to membrane disruption by freeze-thaw cycles and accessibility of proteins to Proteinase K examined by digestion followed by immunobloting. c. Immunoblot analysis of membranous organelles from U937 cells separated by isopycnic sucrose density gradient centrifugation.