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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Nat Cell Biol. 2010 Nov 21;12(12):1154–1165. doi: 10.1038/ncb2119

Fig. 4. Relationship between mitochondrial fission and autophagy and roles of IRGM, DRP1 and FIS1 in autophagic control of mycobacteria.

Fig. 4

a and b. (a) U937 cells were transfected with GFP-LC3 and siRNA to FIS1, DRP1, IRGM or control and autophagy was induced with hIFN-γ (300 u/ml) for 24 h, and (b) LC3 puncta per cell quantified. c. As in (b) except that autophagy was induced by starvation (4 h). d. LC3 puncta per cell were quantified in cells transfected with either control or MFN1&2 siRNA. e. siRNA treated cells were infected with BCG, autophagy induced by starvation and phagosome maturation \ analyzed using LysoTracker (LTR), as a reporter of acidification 8, 20. f siRNA treated U937 were infected with virulent M. tuberculosis H37Rv, autophagy induced by starvation (4 h) or kept in full medium. CFU were counted to assess bacterial survival. Data, means ± SEM (n=6; two independent transfections, 6 independent infections; SD values are given in Supplementary Table S1). *P<0.05, **P<0.01, †P>0.05 (t-test).