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. Author manuscript; available in PMC: 2010 Dec 2.
Published in final edited form as: Thromb Haemost. 2010 Sep 13;104(6):1116–1123. doi: 10.1160/TH10.1160/TH10-03-0186

Figure 1. NP2 binds to rat Factor IX and Factor IXa.

Figure 1

Typical sensorgrams of NP2 interaction with Factor IXa (A) and Factor IX (B). Different concentrations of FIXa (in nM:a, 20; b, 40; c, 80; d, 160; and e, 320) or FIX (in nM: a, 10; b, 20; c, 40; d, 80; e, 160; f, 320; g, 640) were injected over immobilized NP2 for 180 s. Dissociation of NP2-FIXa and NP2-FIX complex was monitored for 900 s and 800 s, respectively, and a global two-state binding model was used to calculate kinetic parameters. Representative sensorgrams are shown.