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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Mol Cell Biochem. 2010 Oct 5;346(1-2):69–79. doi: 10.1007/s11010-010-0592-5

Fig. 3. The glucocorticoid receptor, C/EBPβ, and C/EBPδ regulate the dexamethasone-mediated increase in 11β-HSD1 expression.

Fig. 3

Human coronary artery smooth muscle cells (HCSMC) were transfected with an siRNA to decrease glucocorticoid receptor (GR) expression or a scrambled control (SS), treated with dexamethasone (10−7 mol/L), and (a) 11β-HSD1 mRNA levels were determined by qRT-PCR (n=3) and (b) protein expression by Western immunoblotting (n=3). HCSMC were transfected with an siRNA to decrease C/EBPβ or C/EBPδ expression, respectively, or a scrambled control, treated with dexamethasone (10−7 mol/L), and (c) 11β-HSD1 mRNA levels were determined by qRT-PCR (n=3) and (d) protein expression by Western immunoblotting (n=3). mRNA and protein levels were determined. Data are presented as mean ± SEM and representative blots are shown. *p<0.001 vs. SS, **p<0.001 vs. SS + Dex