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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Mol Cell Biochem. 2010 Oct 5;346(1-2):69–79. doi: 10.1007/s11010-010-0592-5

Fig. 4. 11β-HSD1 regulates glucocorticoid receptor expression.

Fig. 4

HCSMC were transfected with an siRNA to decrease 11β-HSD1 expression, treated with dexamethasone (10−7 mol/L), and glucocorticoid receptor (GR) (a) protein expression was determined by Western immunoblotting (n=3). (b) Protein kinase A (PKA) and phospho-PKA expression were assessed by Western blotting (n=3), and (c) PKA activity was measured (n=4). (d) Expression of the cyclic AMP response element binding protein (CREB) and phospho-CREB were assayed by Western blotting (n=3). Data are presented as mean ± SEM and representative blots are shown. *p<0.001 vs. SS; **p<0.001 vs. HSD1 siRNA; #p<0.05 vs. SS