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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Mol Cell Biochem. 2010 Oct 5;346(1-2):69–79. doi: 10.1007/s11010-010-0592-5

Fig. 7. 11β-HSD1 influences the expression of cell cycle regulators in response to PDGF and dexamethasone.

Fig. 7

Human coronary artery smooth muscle cells (HCSMC) were transfected with an siRNA to decrease 11β-HSD1 expression, synchronized by serum starvation (0.2% serum) for 48 h, and stimulated with 5% fetal calf serum and platelet-derived growth factor-BB (PDGF) (10 ng/ml) in the presence or absence of dexamethasone (10−7 mol/L). Expression of (a) p27kip1 (n=3) and (b) phosphorylation of retinoblastoma protein (phospho-Rb) at Ser608 (n=3) were determined by Western immunoblotting. Representative blots are shown