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. 2010 Nov;131(3):331–339. doi: 10.1111/j.1365-2567.2010.03303.x

Figure 1.

Figure 1

Reactive oxygen species (ROS) production in human umbilical vein endothelial cells (HUVECs) stimulated with lipopolysaccharide (LPS) or interleukin-8 (IL-8). ROS production was assessed by flow cytometry using DCFH-DA in HUVECs stimulated with LPS (a–c) or IL-8 (d–f). (a) Representative data of ROS production in LPS-stimulated HUVECs. (b) Representative data of ROS production in LPS-stimulated HUVECs pre-treated with DPI, an inhibitor of NADPH-oxidase (Nox). (c) Quantitative analysis of ROS production in HUVECs 1 hr (left) or 4 hr (right) after LPS stimulation using relative mean fluorescence intensity (MFI). (d) Representative data of ROS production in IL-8-stimulated HUVECs. (e) Quantitative analysis of ROS production in IL-8-stimulated HUVECs pre-treated with DPI. (f) Quantitative analysis of ROS production in HUVECs 1 hr (left) or 2 hr (right) after IL-8 stimulation. In (d–f) the data represent mean values ± SD (n = 4, **P < 0·01, Student's t-test).