Figure 2.

Tumour necrosis factor receptor 1 deficient (TNFR1^−/−) macrophages have a less inhibitory phenotype than wild-type (WT) macrophages. WT or TNFR1^−/− bone-marrow derived macrophages (BM-Mϕ) were co-cultured with OT-II T cells with 10 μg/ml ovalbumin (OVA) for 72 hr, then surface stained CD4, CD11b, CD31 and Gr-1 (a) and stained intracellularly with anti-TCRξ monoclonal antibody (b). Gr-1 up-regulation was greater with WT Mϕ while CD3ξ expression was reduced compared with naive OT-II CD4⁺ T cells (grey filled) and OT-II CD4⁺ T cells co-cultured for 72 hr with TNFR1^−/− Mϕ (grey lines). These data are representative of three independent experiments.