Figure 3.

T cells regain the ability to proliferate when removed from macrophages. Wild-type (WT) or tumour necrosis factor receptor 1 deficient (TNFR1^−/−) bone-marrow-derived macrophages (BM-Mϕ) were co-cultured with OT-II T cells in the presence of ovalbumin (OVA) peptide. After 24 hr, non-adherent cells were transferred to fresh plates (re-plated) and all cells were washed into fresh media. Proliferation was measured after a further 48 hrs of culture for control cells (solid lines) and re-plated cells (broken lines) for cultures initially containing either WT macrophages (black lines) or TNFR1^−/− macrophages (grey lines). These data are representative of four independent experiments.