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. 2010 Nov;131(3):350–356. doi: 10.1111/j.1365-2567.2010.03306.x

Figure 2.

Figure 2

The stimulation of APRIL induced the expression of matrix metalloproteinase 9 (MMP-9) and interleukin-8 (IL-8) in THP-1 cells. THP-1 cells were stimulated with 1, 3, or 10 μg/ml concentrations of TACI : Fc or anti-APRIL monoclonal antibody (mAb) for 24 hr and the culture supernatant was collected. Gelatin zymogram was performed to analyse the expression of MMP-9 (a) and enzyme-linked immunosorbent assay was performed to measure the concentrations of IL-8 (b). (c) No treatment control; L, lipopolysaccharide at 1 μg/ml; H, human immunoglobulin G (IgG; 10 μg/ml), M, mouse IgG (10 μg/ml). (c, d) THP-1 cells transfected with small interfering RNA were stimulated with 1 μg/ml of anti-BAFF mAb (b), anti-APRIL mAb (a), or isotype-matching mouse IgG (M) for 24 hr. Culture supernatants were then collected for the measurement of MMP-9 and IL-8. Results are representative for three independent experiments.