Increased expression of cytokines and chemokines in Dusp1−/− mice after colon ascendens stent peritonitis (CASP). Mice were subjected to the CASP procedure and killed 12 hr later. Serum levels of cytokines were determined by enzyme-linked immunosorbent assay. (a) Interleukin-6 (IL-6) and IL-10 levels after 18G CASP. Mice were on mixed 129 × C57BL/6 background, heterozygote littermate controls; data are pooled from two experiments, mean and SEM (n = 10). (b) IL-6 and IL-10 after 20G CASP. Mice backcrossed to C57BL/6, controls were from Harlan (n = 10) or wild-type (WT) littermates (n = 5). Data are pooled from three experiments. (c) Changes in expression of Dusp1 in spleen and liver 12 hr after 20G CASP. (d, e) Changes in gene expression in spleen (d) or liver (e) 12 hr after 20G CASP. (c–e) Data are pooled from two experiments, mean and SEM (n = 10 for each genotype for treated, n = 2 for untreated control mice). Asterisks indicate P < 0.05 in Student's t-test.