Table 1.

Gene-specific primers and probe combinations assayed by quantitative real-time polymerase chain reaction

Gene ID	NCBI ID		Primer sequence 5′-3′	Probe
β-actin	NM_007393·3	L	ctaaggccaaccgtgaaaag	64
		R	accagaggcatacagggaca
socs1	NM_009896·2	L	gtggttgtggagggtgagat	20
		R	cctgagaggtgggatgagg
irak3	NM_028679·3	L	ggacattcgaaaccaagcat	2
		R	ccagtgcttctttagttgcattt
sigirr	NM_023059·3	L	ggtggcagtcgtgactcag	72
		R	acctcaggagagagcagttagc
tmed1	NM_010744·3	L	gctagtcttgagaccgagtacca	26
		R	gctctccaaggtgaagtcca
irak1	NM_008363·2	L	ggatcagctccaccttcaga	32
		R	cccagaagaatgtccagtcg
tollip	NM_023764·3	L	gcagggtgttggctatgtg	40
		R	cattacagcggggctgag
tlr1	NM_030682·1	L	tcttgctggcacccattc	58
		R	catgagagttttgagcttgtgg
tlr2	NM_011905·3	L	ggggcttcacttctctgctt	50
		R	agcatcctctgagatttgacg
tlr3	NM_126166·4	L	gatacagggattgcacccata	26
		R	tcccccaaaggagtacattaga
tlr4	NM_021297·2	L	ggactctgatcatggcactg	2
		R	ctgatccatgcattggtaggt
tlr5	NM_016928·2	L	tcatggatggatgctgagtt	18
		R	tggccatgaagatcacacc
tlr6	NM_011604·3	L	ggtaccgtcagtgctggaa	110
		R	gggttttctgtcttggctca
tlr7	NM_133211·3	L	gatcctggcctatctctgactc	25
		R	cgtgtccacatcgaaaacac
tlr8	NM_133212·2	L	caaacgttttaccttcctttgtc	56
		R	atggaagatggcactggttc
tlr9	NM_031178·2	L	gaatcctccatctcccaacat	79
		R	ccagagtctcagccagcact