Table 1.
Genotyping primers for markers close to the opsin genes. Primer sequences and location relative to the opsins genes are listed.
| Marker | Cross | Location and Primer sequences |
|---|---|---|
| UVusat (TCAA)4 |
Both | Third intron of SWS1 |
| F: GCGCGGAATTCACATCCCTGAAAGTCTGGGC | ||
| R: GCGCGCAAGCTTCTGTTTGTTCATGAAGACGTAGA | ||
| Green2 (GTT)13 |
DC | 4.9 kb from end of RH2B and 3.8 kb from begin of RH2Aα |
| F: TGCCTTTGGTGAGTCTTTTT | ||
| R: CCACATTGGAATAAAGTTCATGTT | ||
| Green1usat2 (CA)43 |
TA | 11.4 kb from end of RH2Aβ |
| F: CCACAATTACACTGCGAAGG | ||
| R: TGAATGGTGTGCCTGTTGTT | ||
| Redblue4 (CT)15 |
DC | 22 kb from end of LWS |
| F: AGTCACAAGCCATAAATACTTGAACA | ||
| R: AATATATTACATTTTCGGTCCGTCTC | ||
| Redblue3 (CA)30 |
TA | 7.6 kb from end of LWS |
| F: TGTTCCATCATTCAACTTCATCA | ||
| R: AAGAACACCATGGCAGGATT |