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. 2010 Nov;162(2):244–250. doi: 10.1111/j.1365-2249.2010.04242.x

Fig. 2.

Fig. 2

Serum amyloid A (SAA) stimulates interleukin (IL)-23 p19 and p40 mRNA expressions in rheumatoid arthritis (RA) synoviocytes. (a) RA synoviocytes were unstimulated or stimulated with SAA (0·5, 5 µg/ml) and lipopolysaccharide (LPS; 100 ng/ml) for 6 h in the presence or absence of polymyxin B (10 µg/ml). IL-23 p19 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression was determined by real-time polymerase chain reaction (PCR). (b) RA synoviocytes were stimulated with SAA (0·5, 5 µg/ml) for 6 h. IL-23 p40 and GAPDH mRNA expression was determined by real-time PCR. (c) RA synoviocytes were stimulated with SAA (0·5, 5 µg/ml) for 6 h. IL-12 p35 and GAPDH mRNA expression was determined by real-time PCR. (d) RA synoviocytes were stimulated with SAA (5 µg/ml) for 0, 3, 6, 9 and 12 h. IL-23 p19 and p40 mRNA expressions were determined by real-time PCR. Data are means of three different RA–fibroblast-like synoviocytes run in triplicate ± standard deviation. *P < 0·0001 compared to untreated RA synoviocytes.