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Comparison of immunofluorescent staining approaches using isolated peripheral blood mononuclear cells or whole blood. The relative frequencies of total B cells (as percentage of all lymphocytes) as well as different B cell subsets (as percentage of all CD19+ B cells) obtained from staining approaches using peripheral blood mononuclear cells (PBMC) or whole blood method were compared. Spearman's r correlation values are shown and all were significant (P < 0·01).
