Fig. 2.

CCR3 is highly expressed in the colon of ulcerative colitis (UC) patients. Total RNA was extracted from colonic biopsies taken from inflammatory bowel disease patients (UC and Crohn's disease (CD)] and normal controls (NCs) and multiplex reverse transcription–polymerase chain reaction (RT–PCR) for CCR3 and glyceraldehyde-3-phosphate-dehydrogenase (GAPDH) was performed. (a) Representative PCR blots of mRNA expression from tissue obtained from Crohn's disease (CD), active (a) and inactive (i) and ulcerative colitis (UC) patients, with active (a) and inactive (i) disease and NCs, probed for CCR3, and GAPDH as internal control. In order to control for genomic contamination each sample has a reverse transcriptase negative control (RT^–). The gel shows fluorescence of ethidium bromide-stained PCR products resolved by electrophoresis. (b) Mean densitometry readings for PCR production of receptors are expressed as a percentage of receptor mRNA expression observed in UC patients' samples. Each column represents mean ± standard error of the mean of the different groups. *P < 0·05 between UC and CD, between CD and NCs and between UC and NCs.