Fig. 5.

Down-regulation of single immunoglobulin (Ig) interleukin (IL)-1R-related molecule (SIGIRR) expression in colonic epithelial cells exposed to tumour necrosis factor (TNF)-α and lipopolysaccharide (LPS). SW480 (a) and human monocytic leukaemia cell line (THP-1) (b) cells (5 × 10⁴ cells/well) were stimulated with TNF-α (1 ng/ml) or LPS (100 ng/ml), after which the gene expressions of SIGIRR and IL-8 at various time-points were examined using real-time polymerase chain reaction (PCR). Error bars indicate the standard error of mean values obtained from three independent experiments. #P < 0·01, *P < 0·05 versus non-stimulated cells.